Fig. 6.

Effect of Cu²⁺ in the growth rate of ctpB-disrupted M. tuberculosis cells. a The growth rate of M. tuberculosis ΔctpB (black) and M. tuberculosis H37Ra wild-type (gray) cells in modified Sauton medium supplemented with high doses of Cu²⁺ was measured at OD₅₉₅. The data are shown as the mean ± SEM from three independent experiments. b Serial dilutions (tenfold) of M. tuberculosis cells (wild type and ΔctpB cells) previously grown until mid-log phase were seeded and grown on 7H11-OADC plates supplemented with 0.25 and 100 μM CuSO₄