Table 3.
Results of S. mansoni and S. haematobium DNA experimental analyses
| Treatment | Replicate code | S. mansoni | S. haematobium | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| eDNA amplification success | eDNA Cq (mean) | eDNA copies/µl (mean) | n/N | Infected snail biomass (g) | eDNA amplification success | eDNA Cq (mean) | eDNA copies/µl (mean) | n/N | Infected snail biomass (g) | ||
| A | 1 | 3/3 | 32.47 | 211.48 | 8/21 | 1.49 | 3/3 | 32.88 | 20.14 | 3/3 | 1.38 |
| 2 | 3/3 | 35.13 | 35.62 | 10/21 | 1.71 | 3/3 | 34.53 | 6.35 | 3/3 | 1.44 | |
| 3 | 3/3 | 33.92 | 72.73 | 6/19 | 0.83 | 2/3 | 36.11 | 2.64 | 2/3 | 1.03 | |
| 4 | 3/3 | 37.11 | 6.71 | 5/20 | 0.59 | 3/3 | 32.36 | 29.30 | 2/3 | 0.76 | |
| 5 | 3/3 | 38.59 | 2.57 | 12/21 | 1.91 | 0/3 | – | 0 | 1/2 | 0.38 | |
| 6 | 3/3 | 38.48 | 2.45 | 3/20 | 0.37 | 2/3 | 37.28 | 1.03 | 0/3 | 0 | |
| B | 7 | 3/3 | 36.55 | 10.20 | 9/20 | 0.56 | 3/3 | 28.91 | 325.97 | 4/6 | 2.07 |
| 8 | 3/3 | 34.47 | 52.78 | 6/20 | 1.07 | 2/3 | 36.28 | 1.95 | 1/3 | 0.33 | |
| 9 | 3/3 | 35.03 | 33.27 | 11/20 | 1.72 | 3/3 | 33.10 | 19.53 | 6/6 | 2.48 | |
| 10 | 3/3 | 33.85 | 77.28 | 7/19 | 0.84 | 3/3 | 29.56 | 222.73 | 4/6 | 1.68 | |
| 11 | 3/3 | 31.47 | 435.27 | 11/21 | 1.2 | 3/3 | 30.07 | 160.39 | 3/6 | 1.17 | |
| 12 | 3/3 | 33.59 | 94.77 | 6/20 | 0.76 | 3/3 | 33.10 | 19.50 | 1/6 | 0.49 | |
| C | 13 | 3/3 | 36.14 | 18.16 | 4/10 | 0.64 | 3/3 | 34.86 | 8.01 | 2/3 | 0.53 |
| 14 | 3/3 | 34.81 | 40.36 | 3/10 | 0.39 | 0/3 | – | 0 | 2/3 | 0.64 | |
| 15 | 3/3 | 39.68 | 1.09 | 4/10 | 0.30 | 3/3 | 37.09 | 1.48 | 3/3 | 0.78 | |
| 16 | 3/3 | 34.05 | 67.49 | 2/11 | 0.14 | 3/3 | 35.05 | 4.95 | 2/3 | 0.59 | |
| 17 | 3/3 | 36.21 | 22.03 | 3/10 | 0.37 | 3/3 | 24.17 | 8445.25 | 3/3 | 0.48 | |
| 18 | 3/3 | 36.03 | 20.46 | 5/10 | 0.54 | 3/3 | 28.77 | 342.91 | 3/3 | 1.20 | |
| D | 19 | 3/3 | 35.03 | 43.35 | 4/10 | 0.66 | 3/3 | 34.29 | 7.61 | 5/5 | 1.23 |
| 20 | 3/3 | 35.89 | 26.35 | 4/10 | 1.13 | 3/3 | 29.65 | 180.51 | 6/6 | 1.44 | |
| 21 | 3/3 | 37.26 | 12.39 | 7/10 | 0.97 | 3/3 | 31.49 | 49.80 | 5/5 | 1.07 | |
| 22 | 3/3 | 35.63 | 27.94 | 6/11 | 1.27 | 3/3 | 27.90 | 611.29 | 6/6 | 1.79 | |
| 23 | 3/3 | 37.95 | 5.25 | 4/10 | 0.58 | 3/3 | 35.36 | 3.49 | 6/6 | 1.44 | |
| 24 | 3/3 | 36.04 | 21.65 | 5/10 | 0.44 | 3/3 | 31.36 | 53.09 | 6/6 | 1.34 | |
| E | 25 | 0/3 | – | – | – | – | 0/3 | – | – | – | – |
| 26 | 0/3 | – | – | – | – | 0/3 | – | – | – | – | |
| 27 | 0/3 | – | – | – | – | 0/3 | – | – | – | – | |
| 28 | 0/3 | – | – | – | – | 0/3 | – | – | – | – | |
| 29 | 0/3 | – | – | – | – | 0/3 | – | – | – | – | |
| 30 | 0/3 | – | – | – | – | 0/3 | – | – | – | – | |
| F | 31 | 0/3 | – | – | – | – | 0/3 | – | – | – | – |
| 32 | 0/3 | – | – | – | – | 0/3 | – | – | – | – | |
Note: The table includes the results from eDNA monitoring and the results of the qPCR tests of host snail infection
Key: –, no amplification was observed
Abbreviations: n, number of infected snails; N, number of all host snails in the assay