Figure 1.

Cell proliferation index (CI) of porcine luminal epithelial cells cultivated for 168 h. The experiment consisted of eight replicates involving the cultivation of the same population of collected cells. The cell proliferation index (CI) was assessed for real-time in vitro cultivation for the time periods 0–24 h (a), 24–48 h (b), 48–72 h (c), 72–96 h (d), 96–120 h (e), 120–144 h (f), and 144–168 h (g). CI is an unitless parameter calculated based on impedance of electron flow caused by adherent cells; CI = (impedance at time point n − impedance in the absence of cell)/nominal impedance value. The differences were considered to be significant at the level of ^∗P < 0.05, ^∗∗P < 0.01, and ^∗∗∗P < 0.001.