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. 2020 Feb 13;30:2. doi: 10.1186/s12610-020-0099-1

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© The Author(s). 2020

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — RISUG: mode of action. (A) Vas is exposed from inguinal region and RISUG is injected in both vas deferens towards distal region by a micro-syringe. (B) RISUG coats the wall of the vas deferens blocking sperm movement. (C) Complete reversal obtained after DMSO/NAHCO₃ is injected bilaterally, flushing component of RISUG [37]