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. 2020 Feb 12;18:24. doi: 10.1186/s12964-019-0485-5

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© The Author(s). 2020

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — DCX Nuclear import is promoted by GDNF. a Immunostaining of GDNF-treated GDCX and GDCXU251 stem cells compared with untreated GDCX cells. Scale bar, 20 μM. b Western blotting assay showing DCX nucleocytoplasmic expression in both MARK1-treated and untreated GDCX U251 cells. c Representative bioluminescence images comparing GDNF-treated GDCXC6 cells, GDNF-treated-wt C6, and untreated wt C6 cells at week 3 post-implantation. d Immunostaining of brain sections showing DCX nuclear accumulation intensity. Scale bars = 25 μm. Immunofluorescence image of invasive glioma markers, CD31 (E), and EGFR (F), Scale bars = 25 μm