Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Measurement of CMA in vitro. Top: lysosomes active for CMA can be isolated by flotation in a discontinuous gradient of metrizamide. Bottom: incubation of CMA substrates with intact lysosomes pre-treated or not with inhibitors of lysosomal proteases allows to quantify substrate binding and translocation (uptake) inside the lysosomal lumen
