Skip to main content
. Author manuscript; available in PMC: 2020 Feb 13.
Published in final edited form as: Methods Mol Biol. 2019;1880:703–727. doi: 10.1007/978-1-4939-8873-0_47

Table 1.

Antibodies or CMA-related proteins and recommended dilutions

Antigen Type Source/cat# Dilution (IB) Dilution (IF)
AKT Rabbit pAb IgG Cell signaling [9272] 1:1000 1: 200
pAKT (ser473) Rabbit pAb IgG Cell signaling [9271] 1:1000 1: 25
Cath A (A-19) Goat pAb IgG Santa Cruz [sc-26049] 1:500 N/A
Cath D Goat pAb IgG Santa Cruz [sc-6486] 1:500 N/A
EF1α Mouse mAb IgG Millipore [05–235] 1:1000 N/A
GAPDH Rabbit mAb IgG Cell signaling [2118] 1:1000 1:100
GFAP Mouse mAb IgG Millipore [MAB360] 1:1000 1:200
pGFAP Rabbit pAb IgG ABGENT [AP3562a] 1:1000 N/A
HSC70 (13D3) Mouse mAb IgM Novus biological [NB120–2788] 1:5000 1:500
HSP90 Rat mAb IgG ENZO 1:10,000 N/A
LAMP-1 [H4A3] Mouse mAb IgG Abcam [ab25630] 1:3000 1:100
LAMP2A Rabbit pAb IgG ThermoFisher [51–2200] 1:1000 1:200
mTOR Rabbit pAb IgG Cell signaling [2972] 1:1000 N/A
Rictor Rabbit mAb IgG Cell signaling [2114] 1:1000 N/A
Rac1 Mouse mAb IgG Millipore 1:1000 N/A
Ribonuclease A Rabbit pAb IgG Rockland immunochemicals 1:10,000 N/A

pAb polyclonal antibody, mAb monoclonal antibody