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MY-5445 stimulates ABCG2 ATPase activity. The effect of increasing concentrations of MY-5445 (0-1.5 μM) on the vanadate-sensitive ATPase activity of ABCG2 was measured in the membrane vesicles prepared from High-Five insect cells overexpressing human ABCG2 as previously described [70]. Values represent mean ± SEM calculated from three independent experiments.
