Figure 3.

TFRC knockdown suppresses EOC cell proliferation in vitro and in vivo. A. Flow cytometry for Ki-67 expression in high TFRC expression tissues (TFRC^High) and paired low TFRC expression tissues (TFRC^Low) from EOC patients. B. A CCK-8 assay was performed to evaluate the effect of TFRC knockdown on EOC cell proliferation. C. Cell proliferation rates were determined by EdU assay. D, E. Cell cycle analysis of the NC- or sh1-TFRC-transfected cells. F, G. TFRC knockdown in EOC cells inhibited tumor growth in vivo. All experiments were performed independently at least three times, and the data are expressed as the mean ± SD. *P<0.05, **P<0.01, ***P<0.001 and ****P<0.0001.