Figure 4.

TFRC knockdown suppresses EOC cell metastasis in vitro and in vivo. (A, B) A transwell assay was performed to evaluate the effect of TFRC knockdown on EOC cell metastasis (scale bar, 100 μm). (C) TFRC knockdown in SKOV3 cells inhibited tumor metastasis in vivo. Representative images of the whole body, bowel and liver are shown in sequence. (D, E) Statistical analysis results of the data obtained in (B) are shown. (F) H&E staining was performed to confirm the metastatic tumors (scale bar, 1 nm). (G) The metastasis of A2780 cells in vivo was monitored by bioluminescence imaging. Whole-body images of mice and statistical analysis results are shown. (H) Representative fluorescence images of the bowel/liver and statistical analysis results are shown. All experiments were performed independently at least three times, and the data are expressed as the mean ± SD. *P<0.05, **P<0.01, ***P<0.001 and ****P<0.0001.