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. 2020 Jan 1;10(1):224–236.

Figure 3.

Figure 3

SIX4 regulates invasion-metastasis cascade through EMT program. A. The mRNA expression of EMT-TFs and EMT markers were determined in MDA-MB-231 cells silenced with control (Sh-NC) or SIX4 Sh-RNA (no. 1 and 2). B. MDA-MB-231 cells were silenced with control (Sh-NC) or SIX4 Sh-RNA (no. 1 and 2), and whole-cell extracts were collected for IB analysis. C. The mRNA expression of EMT-TFs and EMT markers were determined in MCF-7 cells overexpressed with Vector or SIX4. D. MCF-7 cells were overexpressed with Vector or SIX4, and whole-cell extracts were collected for IB analysis. E. Immunofluorescence staining of E-cadherin and Vimentin in paired MDA-MB-231/Sh-NC, MDA-MB-231/Sh-SIX4 cells. F. Immunofluorescence staining of E-cadherin and Vimentin in paired MCF-7/Vector and MCF-7/SIX4 cells. Nuclei were visualized with DAPI staining (blue). Each bar represents the mean ± SD of three independent experiments. Scale bar, 50 μm. ***P<0.001, **P<0.01, *P<0.05.