Upregulation of EPS8L3 expression in human tumor tissues. A. Schematic representation of human EPS8 and three related proteins EPS8L1, EPS8L2 and EPS8L3. B. The mRNA expression of EPS8, EPS8L1, EPS8L2 and EPS8L3 of human liver tissues in TCGA and GTEx databases (Number of tumor tissues = 369; Number of normal tissues = 160). C. The mRNA expression of EPS8L3 was detected in 51-paried (HCC) and 92-paried (ICC) tumor tissues and adjacent non-tumorous tissues by RT-qPCR. D. The protein levels of EPS8L3 were examined by western blotting in 8-paired HCC tissues. E. Representative images of IHC for EPS8L3 expression in tumor tissues and adjacent non-tumor tissues of HCC patients (N = 114). F. HCC patients with low EPS8L3 mRNA expression exhibited better overall survival rate and disease free survival rate in GEPIA database (N = 182 per group). G. Total samples tested and samples with EPS8L3 point mutations in pan-cancer and liver cancer. H. Graphical view of point mutations across EPS8L3 in liver cancer samples. Mutations are displayed at the amino acid level across the full length of the gene. I. Total samples tested and samples with EPS8L3 variants, including copy number variation, gene overexpression and methylation.