Figure 3.

PLCε mediates serine/glycine metabolism and proliferation by modulating YAP. (A, B) Protein level verification of vector-YAP by western blot. (C) mRNA of vector-YAP by q-PCR. (D) q-PCR detection of mRNA levels of PLCε, YAP, PSAT1, PSPH, SHMT2, CyclinD1, and PCNA in cells after infected with vector-YAP or vector-sh-YAP. (E, F) Western blot detected and analyzed the protein expression of these gene mentioned in (D). (G, H) Clonal formation assay (G) and statistical analysis (H) of the numbers of colonies in cells after addition of vector-YAP or sh-YAP plasmid. β-actin were used as internal controls. Data were represented as mean ± SD of three individual experiments. *P<0.05, **P<0.01, and ***P<0.001 vs. controls.