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PLCε regulates dephosphorylation and nuclear translocation of YAP. (A-D) Western blot (A) and protein quantification analyses (B-D) detected the expression of YAP in nucleus and p-YAP in cytoplasm. β-actin and Histone (H) 3 were used as an internal control. (E) Immunofluorescence staining demonstrated YAP intracellular distribution in PCa cell lines. Magnification × 400. Data were represented as mean ± SD of three individual experiments. *P<0.05, **P<0.01, and ***P<0.001 vs. controls.
