Figure 5.

VP enhances the inhibitory effect of PLCε depletion on PCa in vitro. (A) CCK-8 assay to detect the toxicity of different concentrations of VP (2.5 μM, 5.0 μM, 7.5 μM, 10 μM) on PC3 cells. (B-E) mRNA (B) and protein expression (C-E) of PLCε, YAP, PSAT1, PSPH, SHMT2, CyclinD1, and PCNA in the cell lines after treated with VP (5 μM, 48 h). (F-H) Flow cytometry detected cell cycle after cultured with VP (F), and statistical analysis (G, H). (I, J) Mass spectrometry tested the effect of VP on the concentration of serine (I) and glycine (J) in cells. Data were represented as mean ± SD of three individual experiments. *P<0.05, **P<0.01, and ***P<0.001 vs. controls.