Figure 3.
MiR-1246 increases NSCLC cell radioresistance in vitro and in vivo. A. miR-1246 was upregulated after an agomir was transfected into A549 and PC9 cells, while it was downregulated after an antagomir was transfected into A549-R and PC9-R cells. Relative miRNA-1246 expression was detected by qRT-PCR (n=3). B. After transfection, cells were irradiated with 0, 2, 4, 6, 8, or 10 Gy, and colonies formed after 14 days as indicated; n=3 per group. C. Nude mice were subcutaneously injected with tumour cells (n=6) and treated with agomir/nc or antagomir/nc. Tumour volume data are presented as growth curves. When the average volume reached approximately 100 mm3, we radiated the tumours with a single 10 Gy dose. The mice were killed when the longest dimension reached 1.5 cm in any group. Then, the tumours were removed and compared. Data are presented as the mean ± SD. *, P<0.05; **, P<0.01; ***, P<0.001; ns, no significant. Ago: agomir, anta: antagomir, nc: negative control.