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. 2020 Jan 1;10(1):12–37.

Figure 3.

Figure 3

PDAC cell-derived sEVs promotes M2 polarization in THP-1/U937 derived macrophages. A. ELISA analysis of M1 cytokines (TNF-α and IL-1-β, left-panel) and M2 cytokines (CCL18 and IL10, right panel) in THP-1/U937 derived macrophages incubated with PDAC cells (PC080 and PC084)-derived sEVs or HPDE cell-derived sEVs for 72 hrs. B. Flow cytometry analysis of M1 cell surface markers (CD80 and CD86, left-panel) and M2 (CD163 and CD206, right panel) cell surface markers on THP-1/U937 derived macrophages incubated with PDAC cells (PC080 and PC084)-derived sEVs or HPDE cell-derived sEVs for 72 hrs. C. ELISA analysis of M1 and M2 cytokines in THP-1/U937 derived macrophages treated with LPS+INF-γ (M1 induction cytokine, left panel) or IL-4+IL-13 (M2 induction cytokine, right panel), co-incubated with sEVs (sEVs derived from either PDAC cell or HPDE cells) for 72 hrs. D. Flow cytometry analysis of M1 and M2 cytokines in THP-1/U937 derived macrophages treated with LPS+INF-γ (M1 induction cytokine, left panel) or IL-4+IL-13 (M2 induction cytokine, right panel), co-incubated with sEVs (sEVs derived from either PDAC cell or HPDE cells) for 72 hrs. Data represented means ± SD from 3 independent experiments. All results are averaged from 3 independent experiments. Data represents means ± S.D. *P<.05, **P<.005, ***P<0.001 by Student’s t-test.