Skip to main content
. 2020 Jan 1;10(1):12–37.

Figure 10.

Figure 10

PC084 EZR-overexpressed sEVs induce M2 macrophage polarization in THP-1/U937 derived macrophages. A. Western blot analysis of expression of EZR in sEVs derived from PC084-EZR or PC084-Vector. B. ELISA analysis of M1 (TNF-α and IL-1-β, left-panel) and M2 cytokines (CCL18 and IL10, right panel) in THP-1/U937 derived macrophages which incubated with PC084-EZR-derived sEVs, PC084-Vector-derived sEVs, or 100 pg/ml EZR recombinant protein for 72 hrs. Data represented means ± SD from 3 independent experiments (each experiment contains 2 technical replicates). C. Flow cytometry analysis of M1 (CD80 and CD86, left-panel) and M2 (CD163 and CD206, right panel) cell surface markers on THP-1/U937 derived macrophages co-incubated with PC084-EZR-derived sEVs, PC084-Vector-derived sEVs, or 100 pg/ml EZR recombinant protein for 72 hrs. Data represented means ± SD from 3 independent experiments. D. ELISA analysis of M1 or M2 cytokines in THP-1/U937 derived macrophages treated with LPS+INF-γ (M1 induction cytokine, left panel) or IL-4+IL-13 (M2 induction cytokine, right panel) co-incubated with 100 pg/ml EZR recombinant protein, sEVs derived from PC084-EZR or PC084-Vector for 72 hrs. Data represented means ± SD from 3 independent experiments (each experiment contains two technical replicates). E. Flow cytometry analysis of M1 and M2 surface markers on THP-1/U937 derived macrophages which treated with LPS+INF-γ (M1 induction cytokine, left panel) or IL-4+IL-13 (M2 induction cytokine, right panel) co-incubated with 100 pg/ml EZR recombinant protein or sEVs derived from PC084-EZR or PC084-Vector for 72 hrs. Data represented means ± SD from 3 independent experiments. Level of significance was determined using Student’s t-test. *P<.05, **P<.005, ***P<0.001.