Figure 6.

Human CD3ε⁺ cells are the primary cell type in peripheral blood of ART^−/− IL2RG^−/Y SCID pigs. Peripheral blood was collected from 6901 (day 0), 6902, and 6903 (Day 1) and stained for various human immune cell markers. Whole human blood was stained as a control to show gating strategy. All cells in circulation were gated from SCID pigs, while only lymphocytes and monocytes were gated from the human sample for the analysis of T, B, and myeloid cells. RBC were also stained separately for human CD47. (A) Presence of human T cells were assessed by staining for human CD45, CD3ε, CD4α, and CD8α. Cells that were CD3ε⁺ (left) were then assessed for CD4α and CD8α expression (right). Human T cells were present in both 6901 and 6903. Single positive CD4α⁺ and CD8α⁺ cells were also present. (B) Human B cells were assessed by staining for hCD45 and hCD20. B cells were not present in any pigs. (C) Human myeloid cells were assessed by staining for hCD45 and hCD33. Myeloid cells were not present in any pigs. (D) RBCs were stained for human CD47 expression to determine if human erythroid lineage differentiated in SCID pigs. No RBCs from SCID pigs expressed hCD47.