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. 2020 Feb 6;11:100. doi: 10.3389/fimmu.2020.00100

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Copyright © 2020 Boettcher, Li, Ahrens, Kiupel, Byrne, Loving, Cino-Ozuna, Wiarda, Adur, Schultz, Swanson, Snella, Ho, Charley, Kiefer, Cunnick, Putz, Dell'Anna, Jens, Sathe, Goldman, Westin, Dekkers, Ross and Tuggle.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Human leukocyte engraftment in bone marrow, liver, spleen, and thymic tissue of in utero injected ART^−/− IL2RG^−/Y SCID pigs. (A) Lymphoid organs from 0-day old (6901) or 7-day old (6902 and 6903) SCID pigs were analyzed for the presence of human leukocytes by staining with human and pig CD45. Bone marrow liver, spleen, and thymic tissue from both 6901 and 6903 contained human CD45⁺ cells. All cells from isolated bone marrow and thymic tissue were stained, while mononuclear cells from spleen and liver were stained. (B) Human CD45⁺ cells in isolated cells from thymic tissue expressed human CD3ε. PBMCs from an adult human were stained as a gating control. Black histogram is gated on hCD45⁺ cells, while gray is hCD45⁻ cells. Human CD4α and CD8α expression was assessed on human CD3ε⁺ cells within the Art^−/− IL2RG^−/Y thymic tissue and human PBMC.