Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
The mutation efficiency of insertion, deletion and replacement of ura3.
| Mutant of ura3 | Number of mutants | Efficiency (%) |
|---|---|---|
| Disruption | 30 | 100 |
| Insertion | 25 | 83.3 |
| Deletion | 4 | 13.3 |
| Replacement | 1 | 3.3 |
sgRNA1 targeting ura3 was delivered into protoplasts of G. lucidum transformant pJW‐EXP‐intron‐opcas9. Thirty individual 5‐FOA‐resistant mutants were assessed for insertion, deletion and replacement efficiency using the PCR and TA‐cloning sequence assay.
