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The gene deletion frequencies of ura3 in the pJW‐EXP‐intron‐opcas9 strain by dual sgRNA.
| Target site | Number of mutants | Frequency (%) |
|---|---|---|
| sgRNA1 | 14 ± 2 | 28.5 ± 4 |
| sgRNA2 | 17 ± 1 | 34.6 ± 2 |
| sgRNA1 and sgRNA2 | 18 ± 3 | 36.7 ± 6 |
Forty‐nine individual 5‐FOA‐resistant mutants were assessed using the PCR and TA‐cloning sequence assay.
