Table 3.

The effect of the insertion of a 5′‐UTR or intron upstream of the Cas9 gene on mutation frequencies.

Organism	Target gene	The insertion sequence	Fold increase in mutation frequency	Reference
Potato	gbss1	OsMac3 5′‐UTR	2.6	Kusano et al. (2018)
Rice	ysa	adh2 5′‐UTR	4.6	Mikami et al. (2015a)
Ganoderma	ura3	gpd intron 1	10.6	This study