Table 1.
Main production parameters obtained with the single‐copy and multicopy clones at different specific growth rates (µ) in fed‐batch cultures. For the comparing rows, cultivation at the lowest µ of the single‐copy clone is taken as reference.
| Single‐copy clone | Multicopy clone | ||||||
|---|---|---|---|---|---|---|---|
|
Nominal µ (h−1) |
0.050 | 0.100 | 0.150 | 0.025 | 0.050 | 0.100 | 0.150 |
|
Experimental µ (h−1) |
0.048 | 0.088 | 0.133 | 0.023 | 0.051 | 0.087 | 0.147 |
|
Product titre (UA ml−1) |
180 | 195 | 372 | 1414 | 1009 | 827 | 600 |
|
Product titre increase (%) |
− | +8% | +107% | +685% | +460% | +359% | +233% |
|
q P (UA gX −1 h−1) |
77 | 149 | 292 | 374 | 550 | 697 | 777 |
|
q P increase (%) |
− | +92% | +277% | +383% | +611% | +800% | +903% |
|
Q P (103 UA l−1 h−1) |
3.3 | 6.3 | 22.9 | 14.6 | 20.1 | 31.1 | 34.9 |
|
Q P increase (%) |
− | +87% | +586% | +337% | +502% | +831% | +943% |
|
Y P/S (103 UA gS −1) |
0.75 | 0.71 | 0.94 | 7.33 | 5.54 | 3.72 | 2.61 |
|
Y P/S increase (%) |
− | −5% | +26% | +880% | +640% | +398% | +248% |
|
Y P/X (103 UA gX −1) |
1.61 | 1.70 | 2.19 | 16.1 | 10.9 | 8.02 | 5.28 |
|
Y P/X increase (%) |
‐ | +6% | +36% | +902% | +577% | +399% | +229% |
µ, specific growth rate; q P , specific product generation rate; Q P, volumetric productivity; Y P/S, overall product‐to‐substrate yield; Y P/X, overall product‐to‐biomass yield.