Figure 2.

Biomass generation and amounts of secreted recombinant and native proteins in methanol‐ and glucose‐limited fed‐batch (FB) cultivations of the P. pastoris strain producing CpB under control of P_GAP.

A. Biomass generation.

B. Secreted CpB concentrations determined by enzyme activity assay. Average values and standard deviations of the three biological replicates per cultivation condition are shown.

C. Relative transcript levels of TDH3 and CpB in glucose‐ (D) or methanol‐grown cultures (M). Normalized mean expression data from microarray analysis of the three biological replicates per cultivation condition are shown (data taken from Burgard et al. (2017)). All data points are relative to the batch condition.

D. Total secreted protein content in the supernatants during the fed‐batch processes.