Fig. 6. Intracellular pH tracking in Vero cells. Confocal micrographs of cells incubated with pyranine (10 μM) and TmR4C (10 μM) for 30 min in HKR buffer. (a) DIC. (b) Channel (λexc = 405 nm and λem = 525/50 nm) corresponding to the “protonated form” showing higher intensity in the endosomes. (c) Channel (λexc = 488 nm and λem = 525/50 nm) corresponding to the “deprotonated form” showing higher cytosolic intensity. (d) Ratiometric images of Vero cells after cell calibration. Arrows: purple (early endosomes) and yellow (late endosomes).
