Fig. 4. Intracellular localization of GC–NABP nanoprobes in HepG2 cells. HepG2 cells were incubated with GC–NABP nanoprobes at 37 °C for 4 h, and then with 200 μM H2O2 for another 1 h. The endosomes were stained with 1 μM LysoTracker Red for 10 min. Images of HepG2 cells collected from (a) green channel (λex = 488 nm, λem = 500–550 nm) and (b) red channel (λex = 561 nm, λem = 570–620 nm). (c) Bright-field image of HepG2 cells. (d) Merged image of (a), (b) and (c). (e) Fluorescence intensity of HepG2 cells. Location of cells indicated by red line in (d). Scale bar = 10 μm.
