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. 2019 Dec 17;4(2):e10254. doi: 10.1002/jbm4.10254

Figure 4.

Figure 4

Decreased extracellular matrix production with Dot1l deletion in Dot1l Δ/Δ; Acan‐CreER mice. (A) mRNA expression of Acan and Col2a1 in chondrocytes was decreased with Dot1l deletion in Dot1l Δ/Δ; Acan‐CreER mice at 4 weeks of age, after 1 week of deletion (n = 3). Black = control; gray = Dot1l deletion. Chondroitin sulfate immunohistochemical stain of mouse knee joint at (B) 4 weeks of age, 1 week after Dot1l deletion (n = 3), and (C) 15 weeks of age, 12 weeks after Dot1l deletion (n = 4) showed marked decrease in chondroitin sulfate, particularly the articular cartilage SZ. (D) Quantification of chondroitin sulfate signal intensity. Black = control; gray = Dot1l deletion; AC = articular cartilage; SZ = superficial zone; MDZ = middle‐deep zone; GP = growth plate. Scale bar = 100 μm.