Effects of the monophthalate mixture on the sex steroid hormone levels
from antral follicles. Mouse antral follicles were treated with vehicle control
(DMSO) or the monophthalate mixture (0.065–325 μg/mL) for 96
hours. At 24 and 96 hours, media for each treatment-culture were pooled and
subjected to ELISAs for pregnenolone (A), progesterone (B), DHEA (C),
androstenedione (D), testosterone (E), and estradiol (F). The graphs indicate
means ± SEMs from 3–7 separate cultures (8–12
follicles/treatment/culture). * p ≤ 0.05; ^ 0.05 < p ≤
0.10