Effects of the monophthalate mixture on expression levels of cell cycle
regulator genes (CDKI family) and Skp2 from antral follicles.
Mouse antral follicles were treated with vehicle control (DMSO) or the
monophthalate mixture (0.065–325 μg/mL) for 96 hours. At 24 and 96
hours, cDNA samples derived from follicles were subjected to qPCR for
Cdkn1a (A), Cdkn1b (B),
Cdkn1c (C), Cdkn2a (D),
Cdkn2b (E), Cdkn2c (F),
Cdkn2d (G), and Skp2 (H). The graphs
indicate means ± SEMs from 3–7 separate cultures (8–12
follicles/treatment/culture). * p ≤ 0.05; ^ 0.05 < p ≤
0.10