Fig 3. Red1 is required for interaction between Rrp6 and Mmi1.

(A) Co-immunoprecipitation of Rrp6 and Mmi1 in wild-type and red1Δ cells. Native cell extracts were prepared from exponentially growing cells expressing Rrp6-3HA in liquid MM medium and subjected to immunoprecipitation with anti-HA antibody. Precipitates and 10% total cell extracts were then immunoblotted with anti-Mmi1 and anti-HA antibodies. (B) Co-immunoprecipitation of Rrp6 and Mmi1 (left), or Red1 and Mmi1 (right) in wild-type and red1(Δ196–295) cells. Native cell extracts were subjected to immunoprecipitation with anti-HA antibody or anti-GFP antibody. Precipitates and 10% total cell extracts were then immunoblotted with anti-Mmi1, anti-HA and anti-GFP antibodies.