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. 2020 Feb 3;16(2):e1008598. doi: 10.1371/journal.pgen.1008598

Fig 6. Mtl1 mediates interaction between Rrp6 and Mmi1 to eliminate meiotic transcripts.

Fig 6

(A) Localization of Rrp6 in wild-type and mtl1-cs5 cells. Cells expressing Rrp6-mCherry from the endogenous locus were observed during exponential growth at 30˚C in liquid YE medium and shifted to 20°C for 2 hours. Dotted lines indicate the shape of cells. Frequencies of cells containing 0, 1, 2, 3, or 4 and more Rrp6 foci are indicated on the right (n > 100). Scale bar: 5 μm. (B) Expression of mei4 mRNA and ssm4 mRNA in mtl1-cs5 cells expressing Mtl1 and Rrp6-GFP-Mmi1 from plasmids. Cells were grown in liquid MM medium at 30°C and shifted to 20°C for 2 hours. Transcripts were quantified by RT-qPCR and normalized to act1. Error bars represent standard error of three independent samples. *P < 0.05 compared with cells carrying empty vector at 20˚C (Student’s t-test).