Fig 4. Visualization of AaeCad-EGFP in whole mounts of dissected guts from larva, pupa and adult male and female homozygous Aedes.
A. AaeCad protein localization in early 4th instar larval gut. Expression is observed primarily in the gastric caecae and the posterior midgut. B. AaeCad protein expression in pupal gut. C. AaeCad protein localization in adult male gut; D. AaeCad protein localization in adult female gut. Expression in both the male and female was observed primarily in the foregut. Since the tissues were in PBS buffer the samples move ever so slightly under the different filters, preventing use of the merge function. Bar: 50μm.