Skip to main content
. 2020 Feb 3;14(2):e0007948. doi: 10.1371/journal.pntd.0007948

Table 2. CRISPR-Cas9 mediated homologous recombination to generate an EGFP tagged cadherin gene is more efficient with Rad51.

Group Components * Injected embryos G0 larval survivors (%) G0 HR rate (%) G1 HR rate (%)
#1 donor plasmid 512 124 (24.2%) 2/123 (1.6%) N/A
#2 donor plasmid/Rad51 mRNA 516 116 (22.5%) 3/104 (2.9%) 5/65 (7.7%)

* In addition to gRNA and Cas9 protein.