Table 2. CRISPR-Cas9 mediated homologous recombination to generate an EGFP tagged cadherin gene is more efficient with Rad51.
Group | Components * | Injected embryos | G0 larval survivors (%) | G0 HR rate (%) | G1 HR rate (%) |
---|---|---|---|---|---|
#1 | donor plasmid | 512 | 124 (24.2%) | 2/123 (1.6%) | N/A |
#2 | donor plasmid/Rad51 mRNA | 516 | 116 (22.5%) | 3/104 (2.9%) | 5/65 (7.7%) |
* In addition to gRNA and Cas9 protein.