Table 5.

The relationship between cir_ITCH expression level (as divided into two groups based on the median of ΔCt) in GC tissues with clinicopathological parameters.

Name

Sequence (5’--> 3’)

Ta for PCR (°C)

Amplicon size

Divergentprimers

hcircITCH-F1

GTCCGGAACTATGAACAATG

55.9

207 bp

hcircITCH-R1

CTCTGTTGGCTCTTTGTCAC

hcircHIPK3-F1

TATGTTGGTGGATCCTGTTC

55.9

213 bp

hcirc HIPK3-R1

AACTGCTTGGCTCTACTTTG

Convergent primers

hlinITCH-F1

GGTTCACCATCTGCCACTTC

60.7

169 bp

hlinITCH-R1

AGGGAGCTTGAGTTACAGGATT

hlin HIPK3-F1

GAAAGAAACTATCCACGGAC

54.4

177 bp

hlin HIPK3-R1

TATGACCTTTGTAGCACCTG

* A higher ΔCt value indicates higher expression. ** Statistically significant.