Fig. 1.

BMP induces EMT in a spectrum of cancer cell lines. Human ovarian cancer cell line OvCA429, human pancreatic cancer cell line PANC1, and murine mammary cancer cell line Py2T were treated with 10 nM BMP4 for 4 days and a immunostained for phalloidin actin. b mRNA expression levels of SNAI1 and SNAI2 from indicated cells in the presence or absence of 10 nM BMP4 for 24 h analyzed by qRT-PCR. Values are normalized to their untreated controls. c Matrigel transwell invasion in response to BMP4 after 18 h of invasion. Images are of cells on the underside of the filter taken at × 10 and are representative of two independent biological replicates done in triplicate. *p < 0.05, **p < 0.01, ***p < 0.001. Graphs are representative of two independent biological trials done in triplicate for each cell line. (d Indicated cell lines treated with BMP2/4 as in a for indicated numbers of days and analyzed for E-cadherin protein levels by western blots or e by immunofluorescence