Experimental Timeline. Baseline functional testing was performed 2-4 weeks prior to surgery. Additional functional analysis was performed following surgery. Whole body plethysmography was performed at 1,5, and 11 WPS. Videofluoroscopic swallow studies were performed 1, 6, and 12 WPS, and ultrasonic vocalizations were collected at 1,2, and 5 WPS. Laryngoscopy was performed prior to surgical incision, immediately following surgical manipulation of the RLN, and at 13 WPS prior to perfusion for tissue collection. WPS = Weeks post-surgery, WBP = whole body plethysmography, VFSS = videofluoroscopic swallow study, USV = ultrasonic vocalizations.