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. Author manuscript; available in PMC: 2021 Mar 1.
Published in final edited form as: J Comp Neurol. 2019 Oct 18;528(4):574–596. doi: 10.1002/cne.24774

Figure 8.

Figure 8.

Fluorescent immunohistochemistry of the nucleus ambiguus (NA). (a) 40 μm brainstem sections were stained with primary antibody (anti-NeuN; 1:500) followed by an Alexa Fluor 488-conjugated antibody (1:1000). Stereo Investigator software was used to outline the left (arrow heads) and right (arrows) NA with a 2.5x objective that permitted visualization of the entire brainstem section in a single field of view. (b) Neurons with a visible nucleolus (arrows) were counted for each NA (outlined in red) using a 40x objective throughout the entire 40 μm section.