Table 1.

Experimental Methods

Hypothesis	Function Assessed	Methods Used	Time Points	Significant Outcome Metrics
H1	VF Movement	Transoral Laryngoscopy With Automated VF Tracking	Surgery: Pre-incision & Post-Transection 13 WPS	MMRR OCCR Mcorr
H2	Swallowing	Videofluoroscopic Swallow Study	Baseline 1 WPS 6WPS 12 WPS	n/a
H3	Vocalization	Ultrasonic Vocalization Assay	Baseline 1 WPS 2 WPS 5 WPS	High Frequency Modulated Calls Call Bandwidth** Call Duration** Intervocalization Interval
H4	Respiration	Whole Body Plethysmography	Baseline 1 WPS 2 WPS 11 WPS	Tidal Volume*** Minute Ventilation*** Peak Inspiratory Flow*** Apneas: Number & Duration Percent Erratic Breathing
H5	Neuronal Cell Counts*	Immunohistochemistry	13 WPS	n/a

Note: VF = Vocal Fold, WPS = Weeks Post-Surgery, MMRR = Mean Motion Range Ratio, OCCR = Open Close Cycle Ratio, Mcorr = Motion Correlation

^*= Anatomical rather than functional assessment,

^**= affected for a subset of call types,

^***= during respiratory challenge only