. Author manuscript; available in PMC: 2021 Mar 1.

Published in final edited form as: J Comp Neurol. 2019 Oct 18;528(4):574–596. doi: 10.1002/cne.24774

Table 3.

Ultrasonic Vocalization Acoustic Parameter Outcomes

Percentage of Calls (%)
Call Type	Sham (n=10)				Transection (n=9)				F Value	P Value
	Baseline		1 WPS		Baseline		1 WPS
	$\bar{x}$	SD	$\bar{x}$	SD	$\bar{x}$	SD	$\bar{x}$	SD
Simple	38.3	9.8	41.3	13.3	27.7	21.8	27.6	20.2	1.16	0.30
Constant	2.4	3.5	5.1	4.3	4.3	3.4	8.2	11.0	0.46	0.51
Downsweep	3.9	8.0	4.3	9.4	0.7	1.0	0.9	1.2	0.00	0.96
Upsweep	32.0	10.4	31.9	16.4	22.7	18.8	18.6	13.7	1.93	0.18
Jump	35.3	13.2	25.8	8.2	16.5	12.8	24.9	17.1	0.22	0.65
Jump Down	20.5	11.2	17.1	11.1	11.7	12.0	19.2	14.4	2.12	0.17
Jump Up	14.8	15.8	8.6	6.8	4.7	5.3	5.7	5.8	0.37	0.55
Cycle	8.3	7.7	11.4	6.7	12.8	9.2	7.8	7.0	1.45	0.25
Half Cycle	6.1	7.4	7.6	3.9	8.3	9.4	5.3	5.5	0.16	0.70
Full Cycle	1.8	2.2	3.2	2.7	4.2	4.2	2.3	2.2	0.42	0.53
Two Cycles	0.4	0.6	0.5	0.6	0.3	0.6	0.3	0.6	0.20	0.66
Complex	18.1	13.2	21.6	7.5	34.0	28.6	12.4	12.8	0.10	0.76
Multiple Jumps	14.1	11.7	14.2	6.5	29.3	28.7	12.0	12.6	3.74	0.08
Harmonic	3.9	3.4	7.3	4.7	4.6	7.4	0.4	0.7	17.79	0.0007^*
Bandwidth (kHz)
Call Type	Sham (n=10)				Transection (n=9)				F Value	P Value
	Baseline		1 WPS		Baseline		1 WPS
	$\bar{x}$	SD	$\bar{x}$	SD	$\bar{x}$	SD	$\bar{x}$	SD
Simple	17.9	3.7	16.3	4.2	17.9	5.6	10.9	3.2	10.94	0.0048^*
Constant	6.9	3.8	5.2	1.2	6.2	1.8	4.2	0.9	3.90	0.08
Downsweep	18.1	10.5	13.9	8.3	11.5	5.5	10.3	3.1	1.46	0.28
Upsweep	18.0	3.9	17.7	4.2	20.0	5.2	13.0	2.8	11.48	0.0041^*
Jump	25.1	8.0	27.9	5.3	27.7	3.8	24.8	3.7	0.45	0.51
Jump Down	26.3	2.9	28.2	4.7	26.7	3.2	22.1	3.7	6.40	0.026^*
Jump Up	25.0	13.5	28.2	8.2	29.0	5.8	29.9	8.7	0.62	0.45
Cycle	23.4	8.1	21.2	5.9	19.6	7.1	14.0	4.8	4.94	0.046^*
Half Cycle	20.7	5.5	21.7	6.0	19.0	6.1	12.7	5.4	6.41	0.030^*
Full Cycle	23.9	8.3	19.7	7.5	18.6	7.3	14.1	3.8	3.39	0.10
Two Cycles	26.6	12.6	22.7	6.9	19.0	7.6	19.5	3.4	n/a	n/a
Complex	45.0	3.0	44.0	4.9	44.8	5.9	36.7	7.0	2.47	0.14
Multiple Jumps	42.8	5.2	43.7	4.1	44.0	6.7	36.8	6.9	2.83	0.12
Harmonic	47.6	6.8	42.5	13.3	45.6	7.5	39.8	13.8	0.07	0.80
Duration of Call (ms)
Call Type	Sham (n=10)				Transection (n=9)				F Value	P Value
	Baseline		1 WPS		Baseline		1 WPS
	$\bar{x}$	SD	$\bar{x}$	SD	$\bar{x}$	SD	$\bar{x}$	SD
Simple	19.0	5.2	18.2	3.4	17.7	3.3	16.0	2.6	2.02	0.18
Constant	17.0	8.3	17.0	4.2	19.3	9.6	18.0	3.9	0.02	0.89
Downsweep	32.7	28.3	19.5	11.2	15.2	5.6	20.9	9.4	0.32	0.60
Upsweep	18.3	3.2	18.2	3.1	17.3	1.8	14.5	3.2	7.33	0.016^*
Jump	26.5	11.1	29.0	7.1	34.5	10.1	27.7	8.0	0.16	0.70
Jump Down	27.5	4.7	28.2	7.8	35.0	7.3	27.0	7.7	0.08	0.79
Jump Up	20.5	8.5	29.4	7.4	30.3	14.3	27.0	7.7	0.33	0.58
Cycle	45.9	13.1	35.7	9.9	31.0	9.1	41.9	15.8	0.73	0.41
Half Cycle	38.5	10.0	29.9	7.5	27.8	7.5	35.1	15.6	0.75	0.41
Full Cycle	57.2	21.5	46.9	14.5	38.2	13.4	54.5	12.0	0.00	0.97
Two Cycles	70.4	35.8	72.3	31.9	60.8	45.9	127.3	25.7	n/a	n/a
Complex	47.5	9.3	50.5	15.6	50.3	15.8	59.7	14.7	1.15	0.31
Multiple Jumps	44.1	11.0	48.0	11.8	45.2	9.9	59.5	14.6	1.88	0.20
Harmonic	54.6	14.8	54.7	22.9	72.8	36.4	45.0	15.1	1.93	0.21
Peak Frequency of Call (kHz)
Call Type	Sham (n=10)				Transection (n=9)				F Value	P Value
	Baseline		1 WPS		Baseline		1 WPS
	$\bar{x}$	SD	$\bar{x}$	SD	$\bar{x}$	SD	$\bar{x}$	SD
Simple	84.9	5.3	84.1	4.9	83.7	13.1	81.5	4.7	1.28	0.28
Constant	78.0	8.3	75.0	5.4	80.1	3.8	77.9	6.3	0.47	0.51
Downsweep	83.0	9.4	83.2	11.9	89.5	6.4	89.6	10.5	0.89	0.39
Upsweep	85.0	5.5	85.3	5.0	85.1	14.6	83.1	5.3	0.60	0.45
Jump	93.5	8.0	93.4	3.7	94.2	2.0	91.1	3.3	1.14	0.30
Jump Down	95.0	5.1	94.1	5.9	94.7	4.2	88.5	5.1	4.95	0.046^*
Jump Up	93.0	11.0	93.7	7.6	95.9	7.4	94.5	8.4	0.40	0.54
Cycle	90.4	9.5	89.2	4.6	89.1	10.6	83.5	6.2	3.83	0.08
Half Cycle	88.7	6.4	91.0	6.1	91.2	15.1	83.0	8.2	3.72	0.08
Full Cycle	89.2	11.6	85.0	55.5	87.1	8.4	84.8	10.7	0.02	0.90
Two Cycles	89.3	9.4	82.7	7.8	87.2	9.6	84.7	4.2	n/a	n/a
Complex	107.3	3.1	106.7	2.2	105.8	7.5	99.9	7.4	3.82	0.07
Multiple Jumps	106.5	3.9	107.4	4.2	106.1	7.8	100.1	7.1	3.74	0.08
Harmonic	106.4	5.6	101.5	13.6	104.2	8.8	98.8	20.8	0.02	0.90
Duration of Peak Frequency (ms)
Call Type	Sham (n=10)				Transection (n=9)				F Value	P Value
	Baseline		1 WPS		Baseline		1 WPS
	$\bar{x}$	SD	$\bar{x}$	SD	$\bar{x}$	SD	$\bar{x}$	SD
Simple	14.6	4.0	14.4	4.6	14.4	2.4	11.3	2.0	10.11	0.0062^*
Constant	12.5	7.5	10.8	4.6	13.4	6.9	9.2	2.1	1.35	0.28
Downsweep	3.6	2.8	2.8	1.7	1.9	1.3	4.5	2.2	1.44	0.28
Upsweep	16.0	3.2	16.3	3.2	15.2	1.6	12.0	2.7	10.81	0.005^*
Jump	13.9	2.5	14.5	4.4	17.8	11.0	11.6	3.0	1.72	0.21
Jump Down	11.8	4.1	11.3	4.7	12.7	2.7	8.7	3.1	0.80	0.39
Jump Up	16.5	5.4	20.5	5.4	21.5	11.1	18.5	4.1	0.10	0.76
Cycle	23.3	11.0	19.0	5.8	15.5	5.1	17.5	7.1	0.01	0.92
Half Cycle	23.6	10.8	16.8	4.7	14.8	2.8	13.6	5.5	0.33	0.58
Full Cycle	20.9	10.8	25.4	11.9	20.1	7.1	21.1	13.0	0.17	0.69
Two Cycles	18.0	14.5	35.3	30.1	16.2	5.4	46.6	33.8	n/a	n/a
Complex	21.1	5.1	21.4	4.0	21.6	4.1	23.8	8.0	0.71	0.42
Multiple Jumps	19.3	3.9	20.4	4.2	20.7	3.4	23.6	7.7	0.84	0.38
Harmonic	24.0	8.8	22.4	8.8	26.2	5.3	27.9	19.9	0.01	0.91

Note: $\bar{x}$ = mean; SD = standard deviation; WPS = weeks post-surgery; kHz = kilohertz; ms = milliseconds; bold values with an asterisk (*) = statistical significance (p < 0.05) between Sham and Transection groups at 1 WPS. Two mice from the transection group were excluded due to lack of calls. Outcomes could not be assessed for two cycle calls due to a very low percentage of mice with these types of calls.