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. Author manuscript; available in PMC: 2020 Feb 13.
Published in final edited form as: Biochemistry. 2019 Apr 17;58(17):2260–2268. doi: 10.1021/acs.biochem.8b00939

Figure 6.

Figure 6.

Reversibility of 1-methylisatin and AIR reactions via the SAICAR synthetase coupled assay. (A) 1-Methylisatin (500 μM) incubated with excess AIR coupled to the enzymes necessary to convert AIR into the irreversible product SAICAR (Scheme 3). The conversion of the product to the starting material, 1-methylisatin, was monitored by UV–vis at 420 nm. (B) Production of SAICAR from 50 μM AIR was monitored by UV–vis at 282 nm without 1-methylisatin (●) and with 200 μM 1-methylisatin (■). The inset displays the first 5 min of the reaction.