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. Author manuscript; available in PMC: 2020 Feb 13.
Published in final edited form as: Biochemistry. 2019 Apr 17;58(17):2260–2268. doi: 10.1021/acs.biochem.8b00939

Figure 7.

Figure 7.

Determination of the substrate depletion mechanism. (A) Plot of inhibitor, 7-bromoisatin (μM), vs μ, fractional inhibition, of the ATP-NADH coupled assay with various concentrations of AIR: 200 (black circles), 160 (gray circles), 120 (black squares), 80 (gray squares), 40 (black triangles), 20 (gray triangles), and 10 μM (black diamonds) from top to bottom, respectively. (B) Plot of the final slope (y2) from panel A vs AIR (μM).