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. 2020 Feb 13;9:e52378. doi: 10.7554/eLife.52378

Figure 2. β1 spectrin and AnkR rescue Nav channel clustering at nodes in β4 spectrin deficient dorsal roots.

Figure 2.

(A) Immunostaining of dorsal roots from 1.5 month-old Sptbn4F/F and Avil-cre;Sptbn4F/F mice using antibodies against pan-neurofascin (blue), β4 spectrin SD-domain (green), and β1 spectrin (red). Scale bar, 10 μm. (B) Immunostaining of dorsal roots from 1.5 month-old Sptbn4F/F and Avil-cre;Sptbn4F/F mice using antibodies against pan-neurofascin (blue), AnkG (green), and AnkR (red). Scale bar, 10 μm. (C) Quantification of the percentage of dorsal root nodes in Sptbn4F/F and Avil-cre;Sptbn4F/F mice labeled for β1 spectrin, β4 spectrin, AnkG and AnkR. N = 3 mice per genotype with more than 80 nodes counted per data point. Data are mean ± SEM. β1 spectrin, ***p=4.62544E-05; β4 spectrin, ***p=1.97595E-07; AnkG, **p=0.0014; AnkR, ***p=0.0002.

Figure 2—source data 1. Statistical summary for Figure 2C.