Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Mus musculus) | Sptb | https://www.ncbi.nlm.nih.gov/gene/20741 | Gene ID: 20741 | |
| Gene (M. musculus) | Sptbn4 | https://www.ncbi.nlm.nih.gov/gene/80297 | Gene ID: 80297 | |
| Genetic reagent (M. musculus) |
Advillin-cre | F. Wang (Duke University, Durham, NC) | The Jackson Laboratory (Stock No:032536) | |
| Genetic reagent (M. musculus) | Sptbflox/flox | This paper | See Materials and methods, Section Animals | |
| Genetic reagent (M. musculus) | Sptbn4flox/flox | PMID: 30226828 | ||
| Antibody | Anti-Ankyrin G (Mouse monoclonal) | Neuromab | Clone: N106/36; RRID: AB_10673030 | IF (1:500) |
| Antibody | Anti-PanNav (Mouse monoclonal) | Neuromab | Clone: N419/78; RRID: AB_2493099 | IF (1:300) |
| Antibody | Anti-PanNav (Mouse monoclonal) | Sigma-Aldrich | Clone: K58/35; RRID: AB_477552 | IF (1:300) |
| Antibody | Anti-Kv1.2 (Mouse monoclonal) | Neuromab | Clone: K14_16; RRID: AB_2296313 | IF (1:500) |
| Antibody | Anti-β1 spectrin (Mouse monoclonal) | Neuromab | Clone: N385/21; RRID: AB_2315815 | IF (1:500) WB (1:1000) |
| Antibody | Anti-actin (Mouse monoclonal) | Millipore | Cat.#: MAB1501; RRID: AB_2223041 | WB (1:4000) |
| Antibody | Anti-β2 spectrin (Mouse monoclonal) | BD Biosciences | Cat.#: 612563; RRID: AB_399854 | IF (1:1000) |
| Antibody | Anti-Tuj1 (Mouse monoclonal) | BioLegend | Cat.#: 801201; RRID: AB_2313773 | IF (1:800) |
| Antibody | Anti-β3 spectrin (Rabbit polyclonal) | Novus | Cat.#: NB110-58346; RRID: AB_877723 | IF (1:1000) |
| Antibody | Anti-ATF3 (Rabbit polyclonal) | Santa Cruz | Cat.#: SC-188, RRID: AB_2258513 | IF (1:1000) |
| Antibody | Anti-β4 spectrin NT antibody (Rabbit polyclonal) | PMID: 28123356 | RRID: AB_2315634 | IF (1:50) |
| Antibody | Anti-β4 spectrin SD antibody (chicken polyclonal) | PMID: 28123356 | IF (1:200) | |
| Antibody | Anti-β4 spectrin SD antibody (Rabbit polyclonal) | PMID: 28123356 | IF (1:500) WB (1:1000) |
|
| Antibody | Anti-Ankyrin R (Rabbit polyclonal) | PMID: 25362473 | IF (1:500) | |
| Antibody | Anti-Caspr (Rabbit polyclonal) | PMID: 10460258 | RRID: AB_2572297 | IF (1:500) |
| Antibody | Anti-Pan Neurofascin (Chicken polyclonal) | R and D Systems | Cat.#: AF3235; RRID: AB_10890736 | IF (1:500) |
| Sequence-based reagent | Genotyping primer for Sptbn4flox/flox mouse (sense) | PMID: 30226828 | 5’-GAGCTGCATAAGTTCTTCAGCGATGC-3’ | |
| Sequence-based reagent | Genotyping primer for Sptbn4flox/flox mouse (anti-sense) | PMID: 30226828 | 5’-ACCCCATCTCAACTGGCTTTCTTGG-3’ | |
| Sequence-based reagent | Genotyping primer for Sptbflox/flox mouse (sense) | This paper | 5’- ACAGAGACAGATGGCCGAAC-3‘ | |
| Sequence-based reagent | Genotyping primer for Sptbflox/flox mouse (anti-sense) | This paper | 5’-CTCTGGTTCCCAGGAGAGC-3’ | |
| Sequence-based reagent | Genotyping primer for Avil-cre mouse (primer 1) | PMID: 29038243 | 5’-CCCTGTTCACTGTGAGTAGG-3’ | |
| Sequence-based reagent | Genotyping primer for Avil-cre mouse (primer 2) | PMID: 29038243 | 5’- AGTATCTGGTAGGTGCTTCCAG-3’ | |
| Sequence-based reagent | Genotyping primer for Avil-cre mouse (primer 3) | PMID: 29038243 | 5’-GCGATCCCTGAACATGTCCATC-3’ | |
| Software, algorithm | Fiji | National Institutes of Health | RRID:SCR_002285 | |
| Software, algorithm | Qupath | PMID: 29203879 | ||
| Software, algorithm | Prism | Graph Pad | RRID:SCR_002798 | Version 6 |
| Software, algorithm | pClamp | Molecular Devices | RRID:SCR_011323 | |
| Software, algorithm | MultiClamp | Molecular Devices | ||
| Software, algorithm | Clampfit | Molecular Devices |