Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Gene App (Rattus norvegicus) | App, Amyloid beta (A4) precursor protein | http://mar2016.archive.ensembl.org/Rattus_norvegicus/Gene/Summary?g=ENSRNOG00000006997;r=11:24425005-24641858 | Location: Chromosome 11: 24,425,005–24,641,858 |
Mutations were inserted into Exon 16 |
| Genetic reagent (Rattus norvegicus) | Apph | (Tambini et al., 2019) | Rat App allele with humanize Aβ region | |
| Genetic reagent (Rattus norvegicus) | Apps | (Tambini et al., 2019) | Rat App allele with humanize Aβ region and pathogenic Swedish mutations | |
| Genetic reagent (Rattus norvegicus) | Appp | This paper | Rat App allele with humanize Aβ region and protective Icelandic mutation | |
| Recombinant DNA reagent | gRNA1 targeting vector | This paper: http://www.vectorbuilder.com/design/report/733e7a50-b705-45cf-8f8c-8b8206e6f174 | Vector expressing gRNA1 | |
| Recombinant DNA reagent | gRNA2 targeting vector |
This paper http://www.vectorbuilder.com/design/report/2a73df2c-1d5c-4e69-b43a-ee6cfaea80c6 | Vector expressing gRNA1 | |
| Antibody | Y188 anti APP-C terminus (Rabbit polyclonal) | Abcam Cat# ab32136 | RRID:AB_2289606 | WB (1:1000) |
| Antibody | 6E10 anti human APP-Aβ1-16, (Mouse monoclonal) | BioLegend Cat# 803001 | RRID:AB_2564653 | WB (1:1000) |
| Antibody | M3.2 anti rodent APP-Aβ1-16, (Mouse monoclonal) | Biolegend Cat#: 805701 | RRID:AB_2564982 | WB (1:1000) |
| Antibody | Anti-sAPPα C-terminus, (Mouse monoclonal) | IBL Cat# 2B3 | WB (1:1000) | |
| Antibody | Anti-human sAPPβ WT C-terminus (Rabbit polyclonal) | Covance Cat# SIG-39138 | RRID:AB_662870 | WB (1:1000) |
| Antibody | Anti-human sAPPβ Sw C-terminus (Mouse monoclonal) | IBL Cat#: 6A1 | WB (1:1000) | |
| Antibody | Anti-GAPDH (Rabbit polyclonal) | Abcam Cat# ab32136 | RRID:AB_2289606 | WB (1:1000) |
| Antibody | HRP-Anti-mouse antibodies | Southern Biotech Cat#: 1031–05 | RRID:AB_2794307 | WB (1:1000) |
| Antibody | HRP-Anti-rabbit antibodies | Southern Biotech Cat# 4050–05 | RRID:AB_2795955 | WB (1:1000) |
| Antibody | HRP-Anti-rabbit antibodies | Cell Signaling Cat# 7074 | RRID:AB_2099233 | WB (1:1000) |
| Commercial assay or kit | West Dura ECL | Thermo | Cat# PI34076 | |
| Commercial assay or kit | Human β Amyloid (1-42) ELISA Kit – High Sensitive | Wako | ||
| Commercial assay or kit | Human β Amyloid (1-40) ELISA Kit II | Wako | ||
| Commercial assay or kit | V-PLEX Plus Aβ Peptide Panel 1 | Meso Scale Discovery | Cat# K15200G | |
| Commercial assay or kit | V-PLEX Plus Aβ Peptide Panel 1 4G8 | Meso Scale Discovery | Cat# K15199G | |
| Commercial assay or kit | sAPPβ-Sw Elisa), | Meso Scale Discovery | Cat# K151BUE | |
| Commercial assay or kit | WT sAPPα/sAPPβ | Meso Scale Discovery | Cat# K15120E | |
| Other | ChemiDoc MP Imaging System | Biorad | ||
| Instrument/software, algorithm | MESO QuickPlex SQ 120 | Meso Scale Discovery | ||
| Other | xMark Spectrophotometer | Biorad | ||
| Instrument/software, algorithm | Image Lab software | Biorad | RRID:SCR_014210 | |
| Instrument/software, algorithm | GraphPad Prism | RRID:SCR_002798 |