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iSCAT/TIRF microscopy time-lapse movie of unlabeled E254D mutant microtubules (magenta) growing from immobilized microtubule seeds and also spontaneously nucleating in the presence of 12 µM human E254D mutant tubulin and 20 nM human mGFP-EB3 (green) at 30°C. Scale bar is 5 µm. Time stamp is in min:s.
