Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Bos taurus) | ARL6. NCBI Gene ID: 519014 | IDT | - | Codon optimized |
| Biological Sample | Bovine dark-adapted retinas | W L Lawson company (NE, USA) | - | |
| Strain, strain background Escherichia coli cells | BL21(DE3) | Novagen | 69450–4 | Chemically Competent cells |
| Affinity resin | Anti-Flag M2 | Sigma | A2220 | |
| Chemical compound | GTP | Sigma | G8877 | |
| Cryo grids | QUANTIFOIL R 1.2/1.3 | Electron Microscopy Sciences | Q4100AR1.3 | |
| Commercial assay or kit | Gibsons Assembly | Invitrogen | A14606 | |
| Sequence based reagents | Arl6_dN16 Fwd | This paper | PCR primers | GAAGTTCATGTGCTGTGTTTGG |
| Sequence based reagents | Arl6_dN16 Rev | This paper | PCR primers | ACTCCCACCCCCTTTATCATC |
| Sequence based reagents | Arl6_addHis_Fwd | This paper | PCR primers | TG GAA GTT CTG TTC CAG GGG CCC GATTACAAGGACGATGATGATAAAG |
| Sequence based reagents | Arl6_addHis_Rev | This paper | PCR primers | GAATTCTCGAGCGGCCGCCCTTATGTCTTCACCGACTGAATC |
| Software, algorithm | serialEM | doi:10.1038/s41592-019-0396-9 | RRID:SCR_017293 | https://bio3d.colorado.edu/SerialEM |
| Software, algorithm | MotionCor2 v.1.2.1 | doi:10.1038/nmeth.4193 | RRID:SCR_016499 | |
| Software, algorithm | CTFFIND v.4.1.13 | doi:10.1016/j.jsb.2015.08.008 | RRID:SCR_016732 | https://cistem.org/ctffind4 |
| Software, algorithm | RELION v.3.0.4 | doi:10.7554/eLife.42166 | RRID:SCR_016274 | https://www3.mrc-lmb.cam.ac.uk/relion/index.php/Download_%26_install |
| Software, algorithm | Coot v. 0.9-pre | doi:10.1107/S0907444904019158 | RRID:SCR_014222 | https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/ |
| Software, algorithm | Phenix.real_space_refine | doi:10.1107/S2059798318006551 | RRID:SCR_014224 | https://www.phenix-online.org/ |
| Software, algorithm | UCSF Chimera v1.13.1 | doi:10.1002/jcc.20084 | RRID:SCR_004097 | http://plato.cgl.ucsf.edu/chimera/ |
| Software, algorithm | UCSF ChimeraX v.0.9 | doi:10.1002/pro.3235 | RRID:SCR_015872 | https://www.cgl.ucsf.edu/chimerax/ |
| Software, algorithm | PyMOL v2.3.2 | PyMOL Molecular Graphics System, Schrödinger, LLC | RRID:SCR_000305 | http://www.pymol.org/ |
| Software, algorithm | crYOLO | doi:10.1038/s42003-019-0437-z | - | http://sphire.mpg.de/wiki/doku.php?id=pipeline:window:cryolo |
| Software, algorithm | ResMap | doi:10.1038/nmeth.2727 | - | http://resmap.sourceforge.net/ |
| Software, algorithm | I-TASSER | doi:10.1186/1471-2105-9-40 | RRID:SCR_014627 | https://zhanglab.ccmb.med.umich.edu/I-TASSER |
| Software, algorithm | MolProbity v.4.3.1 | doi:10.1107/S0907444909042073 | RRID:SCR_014226 | http://molprobity.biochem.duke.edu |
| Software, algorithm | SBGrid | doi: 10.7554/eLife.01456 | RRID:SCR_003511 | https://sbgrid.org/ |