Table 1. Cryo-EM data collection, refinement and validation statistics.
| BBSome (EMD-21144) (PDB 6VBU) |
BBSome:ARL6:GTP (EMD-21145) (PDB 6VBV) |
|
|---|---|---|
| Data collection and processing | ||
| Magnification | 81,000 | 81,000 |
| Voltage (kV) | 300 | 300 |
| Electron exposure (e–/Å2) | 56 | 56 |
| Defocus range (μm) | −1.1 to −2.4 | −1.1 to −2.4 |
| Pixel size (Å) | 1.06 | 1.06 |
| Symmetry imposed | C1 | C1 |
| Final particle images (no.) | 152,942 | 75,201 |
| Map resolution (Å) FSC threshold |
3.1 0.143 |
3.5 0.143 |
| Refinement | ||
| Resolution limit set in refinement (Å) | 3.1 | 3.5 |
| Map sharpening B factor (Å2) | −45.9 | −43.6 |
| Model composition Non-hydrogen atoms Protein residues Ligands |
30,209 3820 2 Ca2+ |
31,676 4000 2 Ca2+; 1 GTP |
|
B factors (Å2) Protein Ligand |
60.8 74.0 |
53.9 82.3 |
| R.m.s. deviations Bond lengths (Å) Bond angles (°) |
0.005 0.68 |
0.004 0.71 |
| Validation MolProbity score Clashscore Poor rotamers (%) |
2.02 12.0 0.2 |
2.07 12.7 0.7 |
| Ramachandran plot Favored (%) Allowed (%) Disallowed (%) |
93.5 6.5 0.0 |
92.7 7.2 0.1 |