Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Eschericia coli) | BL21(DE3) | Thermo Scientific | EC0114 | competent cells |
| Recombinant DNA reagent | pSMT3-hIMPDH2-WT (pJK053) | https://doi.org/10.1091/mbc.E17-04-0263 | expression plasmid | |
| Recombinant DNA reagent | pSMT3-hIMPDH2-Y12A (pJK061) | https://doi.org/10.1091/mbc.E17-04-0263 | expression plasmid | |
| Peptide, recombinant protein | Ubiquitin-like-specific protease 1 (ULP1) | https://doi.org/10.1091/mbc.E17-04-0263 | purified in house | |
| Peptide, recombinant protein | human IMPDH2 | https://doi.org/10.1091/mbc.E17-04-0263 | purified in house | |
| Chemical compound, drug | LB broth mix | LabExpress | 3003 | bacterial growth media |
| Chemical compound, drug | IPTG | GoldBio | I2481C100 | |
| Chemical compound, drug | MgCl2 | Fisher Scientific | BP215-500 | |
| Chemical compound, drug | KPO4 | Fisher Scientific | BP362-500 | |
| Chemical compound, drug | KCl | Fisher Scientific | BP217-3 | |
| Chemical compound, drug | imidazole | Sigma Aldrich | SLBT7469 | |
| Chemical compound, drug | urea | Fisher Scientific | BP169-212 | |
| Chemical compound, drug | DTT | Fisher Scientific | 172–25 | |
| Chemical compound, drug | HEPES | Fisher Scientific | BP310-1 | |
| Chemical compound, drug | ATP | Sigma Aldrich | A2383-10G | |
| Chemical compound, drug | GTP | Sigma Aldrich | G8877-1G | |
| Chemical compound, drug | IMP | Sigma Aldrich | 57510–5G | |
| Chemical compound, drug | NAD+ | Sigma Aldrich | N6522-1G | |
| Chemical compound, drug | uranyl formate | Electron Microscopy Sciences | 22450 | negative stain EM |
| Software, algorithm | GCTF | https://doi.org/10.1016/j.jsb.2015.11.003 | ||
| Software, algorithm | Relion | https://doi.org/10.7554/eLife.42166 | ||
| Software, algorithm | MotionCor2 | https://doi.org/10.1038/nmeth.4193 | ||
| Software, algorithm | SWISS-MODEL | https://doi.org/10.1093/nar/gky427 | ||
| Software, algorithm | UCSF Chimera | https://doi.org/10.1002/jcc.20084 | ||
| Software, algorithm | COOT | https://doi.org/10.1107/S0907444910007493 | ||
| Software, algorithm | PHENIX | https://doi.org/10.1107/97809553602060000865 | ||
| Software, algorithm | LocScale | https://doi.org/10.7554/eLife.27131 | ||
| Software, algorithm | Molprobity | https://doi.org/10.1107/S0907444909042073 | ||
| Software, algorithm | EMRinger | https://doi.org/10.1038/nmeth.3541 | ||
| Other | C-flat 2/2 holey carbon films on Cu 200 mesh grid | Protochips, Inc | CF-2/2–2C | cryo-EM sample preparation |
| Other | HisTrap FF Crude 5 ml | GE Life Sciences | 17528601 | protein purification |
| Other | Superose 6 Increase 10/300 GL | GE Life Sciences | 29-0916-96 | protein purification |
| Other | Amicon Ulta-15 30K MWCO centrifugal filters | Millipore | UFC903008 | protein concentration |