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. Author manuscript; available in PMC: 2021 Feb 15.
Published in final edited form as: Carbohydr Polym. 2019 Nov 20;230:115651. doi: 10.1016/j.carbpol.2019.115651

Fig. 2. Standard and rapid gas chromatography separation of glucose, G2P, G3P, G6P.

Fig. 2.

(A) Temperature gradient for standard separation of glucose, G3P, and G6P: Initial temperature was 60° C, held for 1 minute, rising at 10° C/minute to 325° C, held for 10 minutes. Total run time: 37.5 minutes. Grey bar indicates window of separation.

(B) Stacked chromatography spectra for silylated glucose, G2P, G3P, and G6P using the temperature setting in (A). Twenty nmoles of each silylated standard were injected into the GC column.

(C) Temperature gradient setting for rapid separation of glucose, G3P, and G6P: initial temperature was 60° C, held for 1 minute, rising at 60° C/minute to 220° C, continued rising at 30° C/minute to 270° C, and finished rising at 30° C/minute to 325° C, held for 5 minutes. Total run time: 12.2 minutes. Grey bar indicates window of separation.

(D) Stacked chromatography spectra for silylated glucose, G2P, G3P, and G6P combined into one sample using the temperature setting in (C). Two nmoles of each silylated standard were injected into the GC column.